Alejo E. Rodriguez-Fraticelli


Head of the Lab of Quantitative Stem Cell Dynamics at IRB Barcelona

CV Summary


I am a developmental and cell biologist interested in tissue regeneration, aging and cancer.

I studied Biochemistry at the Autonomous University of Madrid (UAM). I received my PhD in July 2014 working on mechanisms of epithelial morphogenesis with Dr. Fernando Martin-Belmonte at the "Severo Ochoa" Center for Molecular Biology.

In 2015, I moved to the lab of Dr. Fernando Camargo (Harvard University Department of Stem Cell and Regenerative Biology and Boston Children's Hospital), where I used advanced methods of lineage tracing to study blood stem cell heterogeneity and unveiled a molecular program responsible of long-term self-renewal

During my postdoc, I also worked together with the lab of Dr. Allon Klein (Harvard Medical School Department of Systems Biology) to develop methods to study the gene dynamics of blood stem cell fate determination.   

My work over the years has been supported by many generous foundations and government agencies, including JAE-CSIC, EMBO, LSRF, ASH, LLS and NIH/NHLBI

Since 2021, I head the Lab of Quantitative Stem Cell Dynamics at IRB Barcelona.
Our group focuses on understanding the mechanisms that regulate blood stem cell heterogeneity and how they play a role in homeostasis, immunity and disease. 

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Publications


Single-cell lineage tracing approaches in hematology research: technical considerations


Joana Carrelha, Dawn S Lin, Alejo E Rodriguez-Fraticelli, Tiago C Luis, Adam C Wilkinson, Nina Cabezas-Wallscheid, Cedric S Tremblay, Simon Haas


Experimental hematology, 2020 Sep, pp. 26--36


An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells


Sarah Bowling, Duluxan Sritharan, Fernando G Osorio, Maximilian Nguyen, Priscilla Cheung, Alejo Rodriguez-Fraticelli, Sachin Patel, Wei-Chien Yuan, Yuko Fujiwara, Bin E Li, Stuart H Orkin, Sahand Hormoz, Fernando D Camargo


Cell, 2020 Jun, pp. 1693--1694


Single-cell lineage tracing unveils a role for TCF15 in haematopoiesis


Alejo E Rodriguez-Fraticelli, Caleb S Weinreb, Shou Wen Wang, Rosa P Migueles, Maja Jankovic, Marc Usart, Allon M Klein, Sally Lowell, Fernando D Camargo


Nature, 2020 Jun


Reconstructed Single-Cell Fate Trajectories Define Lineage Plasticity Windows during Differentiation of Human PSC-Derived Distal Lung Progenitors


Killian Hurley, Jun Ding, Carlos Villacorta-Martin, Michael J Herriges, Anjali Jacob, Marall Vedaie, Konstantinos D Alysandratos, Yuliang L Sun, Chieh Lin, Rhiannon B Werder, Jessie Huang, Andrew A Wilson, Aditya Mithal, Gustavo Mostoslavsky, Irene Oglesby, Ignacio S Caballero, Susan H Guttentag, Farida Ahangari, Naftali Kaminski, Alejo Rodriguez-Fraticelli, Fernando Camargo, Ziv Bar-Joseph, Darrell N Kotton


Cell stem cell, 2020 Apr, pp. 593--608.e8


Lineage tracing on transcriptional landscapes links state to fate during differentiation


Caleb S Weinreb, Alejo E Rodriguez-Fraticelli, Fernando D Camargo, Allon M Klein


Science, 2020 Feb, pp. eaaw3381


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Projects


Mechanisms of Stem Cell Memory


Stem cell behavior is defined by their epigenetic status, which can be propagated throughout the clone and defines the molecular properties of their progeny. I study how these stem cell memories arise, how they are modulated upon stimuli, and what are ...


Epigenetic Evolution in Cancer


Cancer cell epigenomes are constantly changing as cells proliferate, acquire new mutations, and experience different environmental stimuli. Some of these differences can be memorized long-term within specific cell clones, and affect the phenotype and p...


Regulation of Hematopoiesis at the Single Cell Level


Every second, millions of blood cells are made in our bone marrow. How is this process regulated to adapt to varying cell demands during injury? How is it quality controlled to avoid dysplasia and cancer?


Methods for Single Cell Lineage Tracing


During my postdoc at Harvard University, I worked on developing methods for single-cell lineage tracing using single cell sequencing platforms. 


Mechanisms of Epithelial Morphogenesis


During my PhD, I have worked on how 3D cues (cell confinement, extracellular matrix composition) translate into changes in gene expression and cell architecture to promote epithelial morphogenesis. As model systems, I used zebrafish and organotypic 3D-...


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Contact


Alejo E. Rodriguez-Fraticelli


Head of the Lab of Quantitative Stem Cell Dynamics at IRB Barcelona


alejo​.rodriguez-fraticelli at irbbarcelona​.org ; alejo​.r​.fraticelli at gmail​.com

+1 6177556007

IRB Barcelona